Effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (Bubalus bubalis) spermatozoa

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Effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (Bubalus bubalis) spermatozoa

AIM The aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (CLC) treated buffalo spermatozoa. MATERIALS AND METHODS Semen samples with mass motility of 3+ and greater, collected from Murrah buffalo bulls were utilized. Immediately after collection, four equal groups of semen sample were made. Group I was kept as control and diluted...

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Effect of egg yolk powder on freezability of Murrah buffalo (Bubalus bubalis) semen

AIM The aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of Murrah buffalo semen. MATERIALS AND METHODS Semen samples (12) from 3 Murrah buffaloes (4 from each bull) with mass motility (≥3+) and total motility (70% and above) were utilized in this study. Immediately after collection, each sample was divided into ...

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Effect of cold shock on enzyme release in buffalo ( Bubalus bubalis ) spermatozoa

We have studied the effect of cold shock on the percentage of enzyme release in buffalo spermatozoa, i.e. alcohol, malic, glucose-6-phosphate, isocitric, lactate, sorbital dehydrogenases, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), hyaluronidase, arylsulphatase A and arylsulphatase B. Significant amounts of alcohol, malic and sorbital dehydrogenases, GOT, hyalu...

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Storage of buffalo (Bubalus bubalis) semen.

Characteristics of buffalo semen, diluents used for liquid storage, aspects involved in freezing and thawing of semen are reviewed, and fertility results after artificial insemination (AI) with frozen-thawed semen are given.

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On the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (Bubalus bubalis).

The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen store...

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ژورنال

عنوان ژورنال: Veterinary World

سال: 2016

ISSN: 0972-8988,2231-0916

DOI: 10.14202/vetworld.2016.182-185